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Related Experiment Video

Updated: May 9, 2025

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
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In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

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In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic

Simran Kumar1, Jenna Venturo1, Helly Patel1

  • 1Department of Biomedical Engineering, University at Buffalo (State University of New York).

Journal of Visualized Experiments : Jove
|May 5, 2025
PubMed
Summary

New methods model early liver organogenesis using human cell lines and assembloid technology. This breakthrough in liver regenerative medicine offers reproducible in vitro models for studying organ development and disease.

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Area of Science:

  • Regenerative Medicine
  • Developmental Biology
  • Bioengineering

Background:

  • Chronic liver disease affects over 800 million globally, with transplantation as the primary treatment.
  • Limitations of current treatments highlight the need for novel therapeutic strategies.
  • Liver organogenesis is crucial for liver development but lacks robust in vitro models.

Purpose of the Study:

  • To develop and present methods for in vitro modeling of early liver organogenesis.
  • To utilize assembloid technology and spheroid cultivation for studying liver development.
  • To identify factors influencing liver organogenesis and cell migration in vitro.

Main Methods:

  • Assembloid technology using hepatic (HEP) and mesenchymal (MES) spheroids to model early liver structures and cell migration.
  • HEP spheroid cultivation systems to study collective cell migration and branching morphogenesis.
  • Use of mesenchymal-conditioned media (M-CM) to induce and analyze branching migration.

Main Results:

  • Successful modeling of early liver organogenesis stages, including morphogenesis and interstitial cell migration.
  • Demonstration of collective migration and branching morphogenesis in HEP spheroid systems.
  • Identification of M-CM's dose-dependent role in initiating branching migration.
  • High reproducibility and success rates across all presented methods.

Conclusions:

  • The developed methods enable reproducible in vitro modeling of key liver organogenesis events.
  • These systems facilitate the study of factors influencing liver development and cell behavior.
  • This work provides a foundation for reverse-engineering organogenesis in vitro and in vivo for regenerative medicine applications.