Related Experiment Video
Updated: May 9, 2025
Orthotopic Implantation and Peripheral Immune Cell Monitoring in the II-45 Syngeneic Rat Mesothelioma Model
Published on: October 2, 2015
Histopathological Evaluation of Mesna Application at Different Concentrations on Middle Ear Mucosa of Rats in Early
E Köroğlu1, S Şirin2, G Turan3
1Department of Otorhinolaryngology, Kocaeli City Hospital, Kocaeli, Turkey.
Background:
The aim of this study was to determine the safe concentration of Mesna, which is widely used in otologic surgeries, by examining the early and late effects on middle ear mucosa, at different concentrations.
Methods:
Fifty-nine healthy ears of 32 rats were included in the study. The rats were divided into eight groups and Mesna at 25%, 50%, and 100% concentrations along with 100% saline were applied. On the third day of the experiment, animals in the first four groups were sacrificed to assess early effects, and on the twentieth day, animals in the last four groups were sacrificed to assess late effects. The middle ear mucosa samples were dissected and delivered blindly to the pathology department.
Results:
Thirty-one rats completed the study. The histopathological effects of Mesna when applied in 25% and 50% concentrations were similar to those of saline in the early period. However, the application of 100% Mesna caused severe inflammation and a statistically significant difference was observed (P = 0.004). Furthermore, vascular proliferation was significant in this group (P = 0.014). There was no significant difference between the groups in terms of late effects.
Conclusion:
In clinical practice, using up to 50% concentration of Mesna can be said to be reliable in obtaining a faster and more efficient chemical dissection. However, an inflammation of the middle ear mucosa was observed in the early period following the application of 100% concentration. Therefore, further studies are needed on its safe use in this concentration.
Insights
Up to 50% Mesna concentration is safe for otologic surgeries, showing no significant middle ear mucosa damage. However, 100% Mesna caused early inflammation, necessitating further research for its safe application.
Area of Science:
- Otolaryngology
- Surgical Innovation
- Histopathology
Background:
- Mesna is frequently utilized in otologic surgical procedures.
- Determining the safe concentration of Mesna is crucial for patient safety.
- Investigating Mesna's effects on middle ear mucosa is essential.
Purpose of the Study:
- To evaluate the safety and effects of varying Mesna concentrations on middle ear mucosa.
- To identify the maximum safe concentration of Mesna for otologic applications.
- To assess both early and late histopathological changes induced by Mesna.
Main Methods:
- A study involving 59 rat ears exposed to Mesna at 25%, 50%, and 100% concentrations, plus saline.
- Early effects were assessed on day 3, and late effects on day 20 post-application.
- Middle ear mucosa samples were histopathologically examined.
Main Results:
- Mesna at 25% and 50% concentrations showed effects comparable to saline.
- 100% Mesna application resulted in significant early inflammation and vascular proliferation (P=0.004, P=0.014).
- No significant differences in late effects were observed across the tested concentrations.
Conclusions:
- Mesna concentrations up to 50% are considered reliable for chemical dissection in otologic surgery.
- High concentration (100%) Mesna induces early middle ear mucosa inflammation.
- Further research is required to establish the safety of 100% Mesna concentration.
