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Updated: May 9, 2025
The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
Published on: April 11, 2014
Protocol to investigate bivalent histone modification dynamics via chromatin immunoprecipitation followed by
1Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712, USA; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA.
Abstract:
Epigenetic modifications play key roles in regulating transcription by altering chromatin structure and accessibility in response to internal cues or external stimuli. Here, we present a protocol for assessing bivalent histone modification changes through chromatin immunoprecipitation (ChIP)-reChIP assay in response to warm ambient temperature in Arabidopsis. We describe steps to perform transient warm ambient temperature treatment on Arabidopsis seedlings. We then detailed procedures for ChIP-reChIP, which can be applied to investigate various DNA-protein interaction dynamics in different experimental contexts. For complete details on the use and execution of this protocol, please refer to Shao et al.1.
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02:36Chromatin Immunoprecipitation- ChIP
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ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
01:20Immunoprecipitation
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...