Parallel assembly of dual-electrochemical cell: a novel approach for simultaneous multiplexed sensing analysis

Area of Science:

• Electrochemistry
• Biosensing
• Chemical Sensing

Background:

• Multiplexed detection of multiple targets in complex matrices is crucial for biosensing and chemical sensing.
• Traditional electrochemical sensing uses multichannel potentiostats for simultaneous analysis.
• Single-electrode methods for multiplexing are limited to specific molecules due to solvent compatibility issues.

Purpose of the Study:

• To introduce a novel electrochemical method, the parallel assembly of a dual-electrochemical cell (PADEC), for simultaneous detection of solvent-incompatible analytes.
• To demonstrate multichannel-like performance using a single-channel potentiostat.
• To overcome limitations of current multiplexed electrochemical sensing techniques.

Main Methods:

• Developed a PADEC system by connecting two distinct electrochemical cells in parallel.
• Enabled concurrent measurement of analytes with different solvent requirements.
• Validated the method by simultaneously quantifying water-soluble vitamin C and lipid-soluble vitamin D3 in their respective optimized media.

Main Results:

• The PADEC approach successfully achieved simultaneous detection and quantification of solvent-incompatible analytes.
• Demonstrated performance comparable to individual detection methods.
• Achieved limits of detection of 27 µM for vitamin C and 32 µM for vitamin D3 over a linear range of 20-400 µM.

Conclusions:

• The PADEC strategy offers a new approach for simultaneous, multiplexed electrochemical determination of analytes in diverse media.
• This method potentially reduces the reliance on expensive multichannel potentiostats.
• The innovation may expand electrochemical applications beyond biosensing to electrocatalysis, energy, and corrosion studies.