Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

Riboswitches

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Predicting the cross-continental spread of the cassava brown streak disease epidemic in sub-Saharan Africa.

Scientific reports·2026

Same author

Effects of acute and chronic gamma irradiations on regeneration of tissue types from selected Ugandan cassava genotypes.

Frontiers in plant science·2026

Same author

Accelerating Resistance Breeding: Emerging Methods to Identify and Validate Plant Immunity Genes.

Plants (Basel, Switzerland)·2026

Same author

Sustainable Insect Pest Management Options for Rice Production in Sub-Saharan Africa.

Insects·2025

Same author

Molecular Diversity and Distribution of Whiteflies (<i>Bemisia tabaci</i>) in Cassava Fields Across South West and North Central, Nigeria.

Insects·2024

Same author

Mycotoxin concentrations in rice are affected by chalkiness, grain shape, processing type, and grain origin.

Mycotoxin research·2024

Search research articles

Related Experiment Video

Updated: May 12, 2025

mRNA Interactome Capture from Plant Protoplasts

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

RNA-Binding Proteome-Wide Analysis Reveals Rice RNA-Binding Proteins Enriched After Sobemovirus Rice Yellow Mottle

Patrick Jacob Odongo^1,2, Roosje Van Ende¹, Sam Balzarini¹

¹Molecular Biotechnology of Plants and Micro-Organisms, Institute of Botany and Microbiology KU Leuven Leuven Belgium.

|May 7, 2025

Summary

Rice yellow mottle virus (RYMV) infection alters host RNA-binding proteins (RBPs). This study identified 11 enriched and 2 depleted RBPs, offering targets for RYMV-resistant rice development.

Keywords:

RBPome RNA binding proteins SAPS Sobemovirus RYMV susceptibility gene viruses

More Related Videos

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

An Assay for Quantifying Protein-RNA Binding in Bacteria

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

Related Experiment Videos

Last Updated: May 12, 2025

mRNA Interactome Capture from Plant Protoplasts

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

An Assay for Quantifying Protein-RNA Binding in Bacteria

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

Area of Science:

Plant Pathology
Molecular Biology
Virology

Background:

Viral RNA interactions with host RNA-binding proteins (RBPs) are critical during infection.
The specific RBPs involved in many viral infections, including Rice yellow mottle virus (RYMV), are not fully understood.
RYMV is a major threat to rice production in Africa, necessitating research into resistance strategies.

Purpose of the Study:

To comprehensively identify host RBPs affected by RYMV infection.
To understand how RYMV infection remodels the host RNA-bound proteome (RBPome).
To discover potential targets for developing RYMV-resistant rice varieties.

Main Methods:

Utilized a silica-based acidic phase separation technique to isolate RBPs.
Performed comparative analysis of the RBPome in RYMV-infected versus uninfected rice.
Quantified changes in non-viral RBP abundance post-infection.

Main Results:

RYMV infection significantly remodels the host RBPome.
Eleven non-viral RBPs were found to be significantly enriched in infected rice.
Two non-viral RBPs showed significantly reduced abundance following RYMV infection.

Conclusions:

This study provides a foundational understanding of RBPome alterations during RYMV infection.
The identified RBPs represent potential candidates for further investigation into RYMV resistance mechanisms.
Further validation of these RBPs is essential for developing RYMV-resistant rice.