Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

16.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.3K
CRISPR01:59

CRISPR

48.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
48.6K
Homologous Recombination02:31

Homologous Recombination

49.8K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
49.8K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Natural Approaches to Appetite Suppression in Obesity: Mechanisms, Evidence, and Clinical Integration.

Nutrition reviews·2026
Same author

Arrhythmias in Dengue: A Systematic Review and Meta-Analysis.

Pathogens (Basel, Switzerland)·2026
Same author

From hepatitis A to acute liver failure in a resource-limited setting: a case report.

IJID regions·2026
Same author

Early Adverse Experiences and the Likelihood of Substance Use Disorders and Non-Fatal Overdose in Clinical and Community Settings: A Systematic Review and Meta-Analysis.

Behavioral sciences (Basel, Switzerland)·2026
Same author

Associations of Depressive Symptom Severity with High-Sensitivity C-Reactive Protein Among U.S. Adults: NHANES 2015-2018.

Journal of clinical medicine·2026
Same author

Urinary Free Cortisol-Based Thresholds for Differentiating ACTH-Dependent Cushing's: A Spanish Validation Study.

The Journal of clinical endocrinology and metabolism·2026

Related Experiment Video

Updated: May 12, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

1.6K

CRISPR-Cas Systems: A Functional Perspective and Innovations.

Carla Navarro1, María P Díaz1, Pablo Duran1

  • 1Endocrine and Metabolic Diseases Research Center, School of Medicine, University of Zulia, Maracaibo 40001, Venezuela.

International Journal of Molecular Sciences
|May 7, 2025
PubMed
Summary
This summary is machine-generated.

Clustered regularly interspaced short palindromic repeats (CRISPR) and associated proteins (Cas) provide prokaryotes with adaptive immunity against viruses. This review details CRISPR-Cas mechanisms and biotechnological applications for future research.

Keywords:
CRISPR-Casgene editinggene therapy

More Related Videos

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

33.6K
Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

27.3K

Related Experiment Videos

Last Updated: May 12, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

1.6K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

33.6K
Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

27.3K

Area of Science:

  • Evolutionary biology
  • Microbiology
  • Immunology

Background:

  • Adaptation is crucial for prokaryotic survival.
  • Clustered regularly interspaced short palindromic repeats (CRISPR) and associated proteins (Cas) form an adaptive immune system in bacteria and archaea.
  • CRISPR-Cas confers resistance to viral infections by integrating viral DNA segments.

Purpose of the Study:

  • To analyze the detailed mechanism of action of CRISPR-Cas systems.
  • To summarize advances in developing CRISPR-Cas-based biotechnological tools.
  • To highlight the therapeutic potential and future research directions of CRISPR-Cas technology.

Main Methods:

  • Literature review of CRISPR-Cas systems.
  • Analysis of molecular mechanisms.
  • Summary of biotechnological applications and advancements.

Main Results:

  • CRISPR-Cas systems provide adaptive immunity through a molecular memory of viral infections.
  • Integration of viral DNA segments enables targeted responses to subsequent challenges.
  • CRISPR-Cas has been developed into novel biotechnological tools with clinical relevance.

Conclusions:

  • A comprehensive understanding of CRISPR-Cas mechanisms is essential.
  • CRISPR-Cas technology offers significant potential for biotechnology and therapeutic applications.
  • Further research is warranted to explore the full capabilities of CRISPR-Cas systems.