Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Spermidine Synthase Gene as a Reporter of Transcription Inhibition in <i>Escherichia coli</i>.

International journal of molecular sciences·2026

Same author

Normalization of GC-MS Metabolomics Data in Adherent Cells: A Practical Comparison of Approaches.

International journal of molecular sciences·2026

Same author

Drug-Metabolizing Gene Expression Identity: Comparison Across Liver Tissues and Model Cell Lines.

Biomedicines·2025

Same author

Contrasting Impacts of Targeted Disruption of the Cancer Stem Cell Marker CD133 and Its Epigenetic Regulator TRIM28 in Colorectal Cancer Cells.

International journal of molecular sciences·2025

Same author

Ovarian Cancer: Multi-Omics Data Integration.

International journal of molecular sciences·2025

Same author

Multiomics of Aging and Aging-Related Diseases.

International journal of molecular sciences·2025

Search research articles

Related Experiment Video

Updated: May 12, 2025

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

Published on: April 10, 2014

Dried Spot Paradigm: Problems and Prospects in Proteomics.

Olga I Kiseleva¹, Yuriy A Ikhalaynen¹, Ilya Y Kurbatov¹

¹Institute of Biomedical Chemistry, Pogodinskaya Street, 10, 119121 Moscow, Russia.

International Journal of Molecular Sciences

|May 7, 2025

Summary

Dried biological fluid proteomics offers advantages for biomarker discovery and diagnostics, especially in remote settings. Addressing challenges in protein stability and standardization is key for clinical adoption.

Keywords:

biomarker diagnostics dried sample proteomics

More Related Videos

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

Published on: April 2, 2018

Related Experiment Videos

Last Updated: May 12, 2025

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

Published on: April 10, 2014

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

Published on: April 2, 2018

Area of Science:

Biochemistry
Analytical Chemistry
Biomarker Discovery

Background:

Biological fluid analysis is vital for diagnostics and precision medicine.
Dried sample matrices (blood spots, saliva, urine) offer convenient collection, storage, and transport.
These matrices are particularly beneficial for remote and resource-limited settings.

Purpose of the Study:

To review the current state of proteomics using dried biological fluids.
To compare the progress of proteomics with other omics approaches (e.g., metabolomics) in this field.
To identify challenges and potential for broader adoption in clinical research.

Main Methods:

Critical review of recent advancements in proteomic methodologies for dried biological fluids.
Comparative analysis of proteomics with metabolomics and other omics in dried matrices.
Examination of protein stability, sensitivity, and standardization issues.

Main Results:

Proteomic analysis of dried biological fluids shows significant potential for biomarker discovery.
Challenges remain in protein stability, assay sensitivity, and standardization across studies.
Dried fluid proteomics complements other omics approaches, offering integrated insights.

Conclusions:

Dried fluid proteomics is a promising tool for non-invasive diagnostics and large-scale epidemiological studies.
Overcoming technical limitations is crucial for widespread clinical and translational research application.
Further development is needed to fully realize the potential of dried matrices in proteomics.