Enhanced Disease Susceptibility1 Regulates Immune Response in Lotus japonicus

Enhanced disease susceptibility1 (EDS1) is a key node in the plant immune signaling network, regulating salicylic acid (SA) levels and other immune responses in Arabidopsis thaliana. We previously reported that modulation of SA by AGD2-like defense response protein 1 (ALD1) has been shown to influence the immune response in Lotus japonicus, but the role of LjEDS1 in this species remains unclear. Here, we identified and characterized the LjEDS1 gene in L. japonicus. The LjEDS1 protein contains a lipase-like domain and an EP domain similar to the Arabidopsis EDS1 protein. Subcellular localization studies revealed that the LjEDS1 protein is distributed in both the cytoplasm and nucleus. Heterologous expression of LjEDS1 in the Arabidopsis ateds1 mutant increased resistance to Pseudomonas syringae pv. Tomato (Pst) strain DC3000. In L. japonicus, roots of the ljeds1 mutants exhibited heightened susceptibility to Ralstonia solanacearum, with increased lesion areas and bacterial titers. Conversely, the overexpression of LjEDS1 reduced the lesion areas and bacterial titers in roots infected with R. solanacearum compared to those in the wild-type. Gene expression analysis showed that LjEDS1 regulates defense-related, basal immunity, and oxidative stress response genes in L. japonicus roots. These findings establish LjEDS1 as an important regulator of disease resistance in L. japonicus.