Related Experiment Video

Updated: May 12, 2025

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
00:07

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.2K

miR-603 mediates thyroid cancer progression by inhibiting HACE1-Dependent YAP1 degradation

Tangya Li1, Jian Chu2, Guangjun Zhou2

  • 1Department of Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, Jiangsu, China; Department of General Surgery, Yancheng First Hospital, Affiliated Hospital of Nanjing University Medical School, Yancheng, 224005, Jiangsu, China.

Archives of Biochemistry and Biophysics
|May 7, 2025
PubMed

Insights

MicroRNA-603 (miR-603) is overexpressed in thyroid cancer (TC), promoting tumor growth by targeting HACE1. This mechanism stabilizes YAP1 protein, driving cancer progression and suggesting new therapeutic strategies.

Area of Science:

  • Molecular biology
  • Oncology
  • Biochemistry

Background:

  • Thyroid cancer (TC) pathogenesis involves complex molecular dysregulation.
  • MicroRNAs (miRNAs) play critical roles in cancer development and progression.
  • Understanding novel regulatory pathways is crucial for identifying therapeutic targets.

Purpose of the Study:

  • To investigate the regulatory role of MicroRNA-603 (miR-603) in thyroid cancer.
  • To elucidate the molecular mechanisms involving HACE1 and YAP1 in TC.
  • To identify potential therapeutic targets for thyroid cancer intervention.

Main Methods:

  • Bioinformatics analysis to identify potential miRNA-target interactions.
  • Dual-luciferase reporter assays to validate miR-603 targeting of HACE1.
  • In vitro cellular assays (proliferation, migration, invasion) and in vivo xenograft models.

Main Results:

  • miR-603 was significantly overexpressed in thyroid cancer tissues and cells.
  • miR-603 directly targets the 3'UTR of HACE1, suppressing its expression.
  • HACE1 suppression by miR-603 leads to YAP1 protein stabilization, promoting TC cell proliferation, migration, and invasion.

Conclusions:

  • A novel regulatory pathway involving miR-603, HACE1, and YAP1 in thyroid cancer pathogenesis was identified.
  • miR-603 promotes tumor growth by stabilizing YAP1 via HACE1 targeting.
  • This pathway represents a potential therapeutic target for thyroid cancer treatment.
Keywords:
E3 ubiquitin ligaseHACE1MiR-603Protein degradationThyroid cancerYes-associated protein 1

More Related Videos

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs10:32

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Published on: December 19, 2019

10.7K
Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

6.7K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
2.9K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.6K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
3.8K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.3K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.7K