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Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

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Cell Labeling with Responsive MRI Contrast Agents is Enabled through Solid-Phase Synthesis.

Liam Connah¹, Danijela Bataveljić², Aleksandra M Bondžić³

¹MR Neuroimaging Agents, Max Planck Institute for Biological Cybernetics, 72076 Tübingen, Germany.

Bioconjugate Chemistry

|May 8, 2025

Summary

This summary is machine-generated.

Researchers developed a novel smart contrast agent (SCA) for magnetic resonance imaging (MRI). This calcium-responsive probe effectively labels and internalizes into cells, overcoming a key limitation in molecular imaging.

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Area of Science:

Biomedical Engineering
Molecular Imaging
Neuroscience

Background:

Smart contrast agents (SCAs) for MRI enable functional molecular imaging by altering signals based on biomarker concentrations.
A significant challenge for SCAs is insufficient accumulation and internalization into target cells for studying intracellular processes.

Purpose of the Study:

To develop a multifunctional SCA capable of cell membrane incorporation and internalization.
To create a calcium-ion-responsive MRI probe for enhanced cellular imaging.

Main Methods:

Solid-phase synthesis was employed to create a trimeric SCA.
A hydrophobic tetradecanoyl group was incorporated to promote interaction with primary rat astrocytes.
The probe's activity, relaxivity changes, cell labeling, internalization, cytotoxicity, and electrophysiological effects were evaluated.

Main Results:

The synthesized SCA demonstrated high calcium-triggered longitudinal and transverse relaxivity changes.
The probe successfully labeled cell membranes and internalized into astroglial cells.
No significant cytotoxicity or adverse effects on cell electrophysiology were observed.

Conclusions:

The developed multifunctional SCA effectively addresses the limitations of cellular uptake and internalization.
This novel probe shows promise for advanced intracellular molecular imaging using MRI.
The agent is suitable for studying intracellular processes in astrocytes without compromising cell viability or function.