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Updated: May 12, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
Complete mitochondrial genome of Ficus hirta and its comparative analysis
Wangdong Deng1,2,3, Xinyi Cai4
1Department of Urology, Longgang District Central Hospital of Shenzhen, Shenzhen, China.
Introduction:
Ficus hirta, known as Wuzhimaotao in China, is a dioecious plant species within the Moraceae family, highly regarded for its medicinal and ecological roles.
Methods:
Utilizing a hybrid assembly methodology, combining Nanopore and Illumina sequencing, we achieved a detailed mitochondrial genomic architecture.
Results:
The genome exhibits single circular structures, spans 486,226 base pairs with 45.21% GC content, and encompasses 31 distinct protein-coding genes.Our analysis extends to gene content, codon usage, intergenomic transfers, phylogenetic relationships, and RNA editing patterns. Notably, the mitochondrial and chloroplast genomes share 15 homologous fragments, underpinning intercellular gene exchange. Phylogenetic positioning confirms Ficus hirta within Moraceae, closely allied with Morus notabilis.
Discussion:
This comprehensive mitochondrial genome elucidation not only augments the biological understanding of Ficus hirta but also enriches genomic resources for future research.
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