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Updated: May 12, 2025

Biological Preparation and Mechanical Technique for Determining Viscoelastic Properties of Zonular Fibers
Published on: December 16, 2021
Influence of zein on viscoelastic properties and gluten network development during dough formation
Tingting Hong1,2,3, Zhiwen Tan3, Dan Xu2,3
1College of Food Science and Engineering, Foshan University, Foshan 528231, Guangdong, PR China.
Abstract:
This study investigated the effects of zein addition on the viscoelastic properties and gluten network of wheat dough. Results from Mixolab and dynamic rheometer showed that 2-3 % zein enhanced dough softness without affecting development time, stability, or creep recovery, while reducing pasting viscosity. Zein increased free water mobility, which decreased dough rigidity and facilitated deformation. Dough with 3 % zein showed a 26.50 % increase in free water relaxation time (T23). SE-HPLC revealed reduced gluten aggregation, increasing SDS-soluble polymeric and monomeric proteins and free sulfhydryl (SH) groups by 20 %. FTIR showed zein promoted antiparallel β-sheets and decreased β-sheet content. TGA indicated a 1.57 % increase in mass loss, and prolonged mixing lowered degradation temperature. CLSM confirmed zein weakened the gluten structure. These findings provided a better understanding of the interactions between zein and gluten in wheat dough, which could help optimize dough processing and improve product quality.
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