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Design and Optimization of the Extraction Process for Eclipta alba Linn. Using Response Surface Methodology.
Priya Dubey1, Maninder K Vasisht1, Subash C Mandal2
1University Institute of Pharmaceutical Sciences -UGC Centre of Advanced Study, Panjab University, Chandigarh 160014, India.
Journal of Chromatographic Science
|May 9, 2025
Summary
Optimizing Eclipta alba extraction using response surface methodology improved wedelolactone content and yield. This enhances the quality and efficiency of this commercially important plant extract.
Area of Science:
- Phytochemistry
- Natural Product Chemistry
- Process Optimization
Background:
- Eclipta alba (Family-Asteraceae) is a key ingredient in many commercial formulations.
- Conventional extraction methods often neglect crucial bioactive marker content.
Purpose of the Study:
- To optimize the extraction process for Eclipta alba using response surface methodology.
- To maximize both extract yield and wedelolactone content.
Main Methods:
- Extraction techniques: Soxhlet, reflux, maceration, ultrasonication.
- Solvents: Acetone, ethanol, 50% aqueous ethanol, 80% aqueous ethanol.
- Optimization variables: Particle size, powder-to-solvent ratio, extraction duration.
- Statistical analysis: Response surface methodology with Box-Behnken design.
Main Results:
- Soxhlet extraction with 50% aqueous ethanol yielded the highest extract (900.04 mg) and wedelolactone (0.4161%).
- Optimal conditions: 4.65 h extraction time, 5.29% w/v powder-to-solvent ratio, 1.19 mm particle size.
- Response surface methodology effectively optimized the extraction parameters.
Conclusions:
- The optimized extraction methodology enhances Eclipta alba extract quality and reproducibility.
- This approach reduces production time and cost for this valuable medicinal plant.
