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The OST1-HOS1-HAT1 module regulates cold response in Arabidopsis thaliana
Xinke Kang1, Fan Wei1, Shuli Chai1
1Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610065, China.
The New Phytologist
|May 9, 2025
Summary
HAT1 acts as a brake on plant cold tolerance by repressing cold-responsive genes. Cold stress triggers OST1 and HOS1 to degrade HAT1, activating plant cold acclimation.
Area of Science:
- Plant Biology
- Molecular Biology
- Stress Physiology
Background:
- Plants possess complex mechanisms for environmental stress adaptation.
- Key regulators like OST1, ICE1, HOS1, and CBFs are known for rapid cold stress response.
- Mechanisms for modulating cold tolerance under fluctuating temperatures are less understood.
Purpose of the Study:
- To elucidate the molecular mechanisms of HAT1 in regulating plant cold stress responses.
- To identify HAT1's cold-dependent posttranslational modification network.
- To understand how plants balance growth and cold tolerance.
Main Methods:
- Multidisciplinary approach: molecular biology, plant physiology, and genetics.
- Analysis of gene expression (CBF and COR genes).
- Investigation of protein-protein interactions and degradation pathways.
Main Results:
- HAT1 represses cold-induced gene expression under normal conditions.
- Cold stress induces OST1-mediated phosphorylation of HAT1.
- HAT1 interacts with HOS1, leading to its ubiquitination and degradation, thereby activating cold response.
Conclusions:
- HAT1 functions as a negative regulator, preventing excessive cold stress response.
- The OST1-HOS1 module controls HAT1 protein stability.
- Plants dynamically balance growth and cold tolerance via HAT1 regulation in response to environmental cues.
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