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A BIN2-SOG1 molecular module regulates replication stress response independently on ATR.
Jie Zhang1, Dongbei Guo1, Yifan Zhang1
1Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Laboratory for Ex Situ Conservation and Resource Utilization of Mountain Plants, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610065, Sichuan, China.
Science Advances
|July 3, 2026
Summary
Plants utilize the BIN2-SOG1 pathway to manage DNA replication stress. BIN2 kinase phosphorylates SOG1, enhancing its stability and transcriptional activity to activate the DNA damage response (DDR).
Area of Science:
- Plant molecular biology
- Genetics and genomics
- Cellular stress response mechanisms
Background:
- DNA replication stress is a significant threat to genome stability in plants.
- SOG1 is a key regulator of the DNA damage response (DDR) in plants.
- While ATM and CRL4PRL1 modifications of SOG1 are known, its regulation during replication stress is poorly understood.
Purpose of the Study:
- To investigate the posttranslational modifications of SOG1 during replication stress.
- To elucidate the role of BIN2 kinase in the plant response to replication stress.
- To identify novel regulatory pathways controlling SOG1 activity and DDR.
Main Methods:
- Analysis of BIN2 kinase activity under replication stress conditions.
- Identification of SOG1 phosphorylation sites by BIN2 using biochemical assays.
- Assessment of SOG1 stability and transcriptional activity following BIN2-mediated phosphorylation.
Main Results:
- BIN2 kinase activity is enhanced during replication stress via an ATR-independent mechanism.
- BIN2 directly phosphorylates SOG1 at threonine residues T244 and T249.
- BIN2-mediated phosphorylation increases SOG1 stability and transcriptional activation of DDR genes.
Conclusions:
- A novel BIN2-SOG1 signaling module regulates the plant response to DNA replication stress.
- BIN2-dependent phosphorylation of SOG1 is crucial for enhancing genome stability under stress.
- This pathway provides a mechanism for dynamic regulation of DDR in plants.
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