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Updated: May 12, 2025
10:06
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
Published on: April 26, 2017
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A Phase-Separated SR Protein Reprograms Host Pre-mRNA Splicing to Enhance Disease Susceptibility
Dong Yan1, Jie Huang2, Fengqi Tian1
1State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
Summary
Plant pathogens manipulate host alternative splicing (AS) to evade immunity. A study identified SR30, a splicing factor, that suppresses tomato immunity by altering defense gene AS, offering new breeding strategies.
Area of Science:
- Plant Molecular Biology
- Plant-Pathogen Interactions
- RNA Splicing
Background:
- Alternative splicing (AS) is crucial in plant-microbe interactions.
- Pathogens exploit host AS to suppress plant immunity.
- Mechanisms of host splicing factor regulation of plant immunity are poorly understood.
Purpose of the Study:
- Identify plant splicing factors regulating immunity.
- Investigate the role of SR30 in tomato immunity against Phytophthora infestans.
- Elucidate the mechanism of SR30-mediated immune regulation.
Main Methods:
- Genome-wide analysis of SR30-governed mRNA AS.
- Investigation of SR30 nuclear condensate formation via liquid-liquid phase separation.
- CRISPR/Cas9 gene editing to knockout SR30 in tomato.
- Assessing disease resistance in SR30 knockout lines.
Main Results:
- SR30 negatively regulates tomato immunity against P. infestans.
- SR30 controls genome-wide mRNA AS and suppresses defense genes.
- SR30 forms nuclear condensates through phase separation, essential for its function.
- SR30 knockout enhances tomato resistance to multiple Phytophthora species by promoting defense gene AS.
Conclusions:
- SR30 is a key regulator of tomato immunity, acting via AS.
- Protein phase separation of SR30 is critical for its immune regulatory function.
- Targeting SR30-mediated AS offers a novel strategy for engineering disease-resistant crops.
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