Related Experiment Video
Updated: May 13, 2025
Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy
Published on: April 13, 2022
Profiling and functional characterization of long noncoding RNAs during human tooth development
Xiuge Gu1, Wei Wei1, Chuan Wu1
1Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Laboratory of Oral Health and Beijing Stomatological Hospital, Department of Biochemistry and Molecular Biology, Capital Medical University School of Basic Medical Sciences, Capital Medical University, Beijing, China.
Long non-coding RNAs (lncRNAs) play a role in human tooth development. Researchers identified specific lncRNAs in dental tissues and found one, DLX6-AS1, promotes odontoblast differentiation, suggesting therapeutic potential for tooth regeneration.
Area of Science:
- Developmental Biology
- Genomics
- RNA Biology
Background:
- Long non-coding RNAs (lncRNAs) are crucial regulators in developmental processes.
- The role and expression dynamics of lncRNAs during human tooth development are not well understood.
Purpose of the Study:
- To investigate lncRNA expression profiles in human fetal tooth development.
- To identify specific lncRNAs involved in odontogenesis and their functional roles.
Main Methods:
- Bulk RNA sequencing of dental epithelium and dental mesenchyme at key developmental stages (bud, cap, bell).
- Single-cell analysis to identify cell-type specific lncRNAs.
- Functional assays to assess the impact of DLX6-AS1 on odontoblastic differentiation.
Main Results:
- Identified lncRNAs co-expressed with developmental regulators, enriched in odontogenesis.
- Discovered specific lncRNAs (e.g., PANCR, MIR205HG, DLX6-AS1, DNM3OS) in dental epithelium and mesenchyme.
- Revealed DLX6-AS1, specific to the coronal dental papilla, enhances odontoblastic differentiation.
Conclusions:
- lncRNAs are significantly involved in human tooth development.
- Specific lncRNAs can serve as developmental markers.
- DLX6-AS1 shows potential as a therapeutic target for tooth regeneration.
More Related Videos
Related Concept Videos
02:39lncRNA - Long Non-coding RNAs
01:15Teeth
In the bud stage, the tooth germ (an aggregation of cells) starts to form in the developing jawbone. During the cap stage, the tooth germ differentiates into enamel organ, dental papilla, and dental sac, which will later develop into the tooth's enamel, dentin...
02:24Ribosome Profiling
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...