Engineered Nanozymes with Asymmetric Mn─O─Ce Sites for Intratumorally Leveraged Multimode Therapy

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Materials Science
• Catalysis

Background:

• Dual-atom nanozymes offer enhanced catalytic activity for tumor therapy due to flexible sites and synergistic effects.
• Precisely regulating diatomic sites' d-band centers to overcome intermediate scaling limitations is a key challenge in nanozyme design.

Purpose of the Study:

• To construct an oxygen vacancies-engineered bimetallic silicate bio-nanoplatform with asymmetric Ce-Mn diatomic catalytic centers.
• To investigate the multimodal synergistic cancer therapy potential of CeMn-V DAs loaded with EGCG and modified with HA.

Main Methods:

• Hydrothermal synthesis of oxygen vacancies-engineered bimetallic silicate bio-nanoplatform.
• Loading epigallocatechin-3-gallate (EGCG) and modification with hyaluronic acid (HA).
• Theoretical calculations to analyze d-band center shifts and electron transport.
• In vitro evaluation of peroxidase-like activity and in vivo tumor inhibition studies.

Main Results:

• Asymmetric Mn-O-Ce moiety optimizes oxygen intermediate adsorption/desorption and enhances peroxidase-like activity.
• Mild photothermal therapy (36.1% efficiency) upon 650 nm laser irradiation inhibits heat shock protein expression.
• Achieved a significant in vivo tumor growth inhibition rate of up to 96.2%.

Conclusions:

• The engineered asymmetric diatomic nanozymes demonstrate superior catalytic and therapeutic efficacy.
• This work promotes the integration of nanotechnology and biology for advanced cancer treatment strategies.