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Updated: May 12, 2025
Detection of Protease Activity by Fluorescent Peptide Zymography
Published on: January 20, 2019
Gelatin Zymography: A Simple and Effective Technique to Detect Gelatinases
Meilang Xue1,2, Haiyan Lin3,4, Christer J Jackson3
1Sutton Arthritis Research Laboratory, Sydney Musculoskeletal Health, Kolling Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia. meilang.xue@sydney.edu.au.
Abstract:
Matrix metalloproteinases (MMPs) are zinc-dependent enzymes capable of breaking down all extracellular matrix (ECM) components. The gelatinases are a subgroup of MMPs which have two members: gelatinase A (MMP-2) and gelatinase B (MMP-9). They are highly efficient in breaking down denatured collagen I or gelatin. This ability of gelatinases has led to the development of a technique called gelatin zymography. This technique provides a simple, sensitive, and robust way to detect gelatinolytic activity in biological samples. This chapter provides a detailed protocol on how to implement the gelatin zymography and analyze gelatinases in biological samples using this technique.
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