Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Combination of 3D and 2D Small and Wide Angle X-Ray Scattering Imaging Reveals Diminished Bone Quality in the Superior Human Femoral Neck Cortex.

Advanced materials (Deerfield Beach, Fla.)·2026

Same author

In situ ptychographic x-ray nanotomography of temperature-controlled crystallization processes.

Nature communications·2026

Same author

In situ ptychographic nanotomography captures activation, mobility, and deactivation of supported catalysts.

Nature communications·2026

Same author

Quantifying Trace Metals in Gunflint Microfossils by 3D Correlative X-ray Nanoimaging.

Analytical chemistry·2026

Same author

Impact of Microporous Layer Composition on the Water Content in the Membrane Electrode Assembly of Polymer Electrolyte Fuel Cells.

ACS applied materials & interfaces·2026

Same author

A fast X-ray shutter for high-power beams.

Journal of synchrotron radiation·2026

Search research articles

Related Experiment Video

Updated: May 15, 2025

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

Cretaceous Chert-Hosted Microfossils Visualized With Synchrotron Ptychographic X-Ray Computed Tomography (PXCT).

Kelsey R Moore^1,2, Theodore M Present¹, Antoine Crémière^1,3

¹Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, California, USA.

|May 11, 2025

Summary

Synchrotron ptychographic X-ray computed tomography (PXCT) reveals new details on how silica preserves microfossils. This study enhances our understanding of ancient microbial life and fossilization processes.

Keywords:

Barra Velha Formation chert microfossil pre‐salt ptychographic X‐ray computed tomography

More Related Videos

Microfocus X-ray CT microCT Imaging of Actinia equina Cnidaria, Harmothoe sp. Annelida, and Xenoturbella japonica Xenacoelomorpha

Microfocus X-ray CT microCT Imaging of Actinia equina Cnidaria, Harmothoe sp. Annelida, and Xenoturbella japonica Xenacoelomorpha

Published on: August 6, 2019

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Published on: February 21, 2017

Related Experiment Videos

Last Updated: May 15, 2025

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

Microfocus X-ray CT microCT Imaging of Actinia equina Cnidaria, Harmothoe sp. Annelida, and Xenoturbella japonica Xenacoelomorpha

Microfocus X-ray CT microCT Imaging of Actinia equina Cnidaria, Harmothoe sp. Annelida, and Xenoturbella japonica Xenacoelomorpha

Published on: August 6, 2019

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Published on: February 21, 2017

Area of Science:

Paleontology
Geochemistry
Microbiology

Background:

Silicification of microfossils is crucial for understanding Earth's ancient microbial ecosystems.
Mechanisms of silica precipitation and organic material preservation in microfossils remain incompletely understood.
Identifying factors contributing to microfossil preservation is key to reconstructing past life.

Purpose of the Study:

To investigate microfossil preservation using synchrotron ptychographic X-ray computed tomography (PXCT).
To characterize diverse morphologies and preservation styles of microfossils from the Cretaceous Barra Velha Formation.
To elucidate silica-organic relationships and taphonomic processes in ancient silicifying environments.

Main Methods:

Application of synchrotron ptychographic X-ray computed tomography (PXCT) for high-resolution imaging.
Generation of 2D and 3D reconstructions of microfossils and silica-organic textures at nanometer resolution.
Analysis of microfossil morphology and preservation variability.

Main Results:

PXCT revealed previously uncharacterized silica textures and organic-silica relationships.
Nanometer-resolution imaging provided insights into microfossil morphologies and preservation styles.
Preservational variability was linked to potential physiological or biochemical differences in ancient microbes.

Conclusions:

PXCT is a powerful tool for analyzing microfossil fine structures and taphonomic processes.
The study enhances understanding of microbial diversity and complexity in the Cretaceous pre-salt basin.
Findings contribute to knowledge of microfossil preservation mechanisms in silicifying environments.