A novel AIEgen-Based probe Integrated with split aptamer Conjugates for adenosine triphosphate detection in beer

Area of Science:

• Biotechnology
• Analytical Chemistry
• Materials Science

Background:

• Adenosine triphosphate (ATP) is a crucial biomarker in biological systems and quality control.
• Existing ATP detection methods often lack the required sensitivity, specificity, or speed.
• Development of novel probes is essential for accurate and efficient ATP quantification.

Purpose of the Study:

• To develop a novel, rapid, specific, and sensitive probe for adenosine triphosphate (ATP) detection.
• To integrate split ATP aptamers with aggregation-induced emission (AIE) for enhanced fluorescence signaling.
• To validate the probe's performance for ATP detection in complex samples like beer.

Main Methods:

• Chemical binding of split ATP aptamers with aggregation-induced emission (AIE) molecules.
• Utilizing aptamer-target binding to induce AIE molecule aggregation and fluorescence enhancement.
• Optimizing experimental conditions for probe performance and validating with beer samples.

Main Results:

• The developed TPE probe demonstrated a linear detection range from 1-1000 nM for ATP.
• Achieved a low limit of detection of 0.14 nM, surpassing previously reported methods in sensitivity and selectivity.
• Showed excellent performance in beer samples with high recovery rates (97.5%-104.6%).

Conclusions:

• The novel TPE probe offers a highly sensitive and specific method for ATP detection.
• The probe's design enables efficient signal transduction through AIE mechanisms.
• This research provides a promising tool for real-time monitoring and quality control in the food and beverage industry, particularly for beer.