Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

446
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
446
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

6.6K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
6.6K
Olefin Metathesis Polymerization: Acyclic Diene Metathesis (ADMET)00:53

Olefin Metathesis Polymerization: Acyclic Diene Metathesis (ADMET)

1.9K
Acyclic diene metathesis polymerization or ADMET polymerization involves cross-metathesis of terminal dienes, such as 1,8-nonadiene, to give linear unsaturated polymer and ethylene. As ADMET is a reversible process, the formed ethylene gas must be removed from the reaction mixture to complete the polymerization process.
Similar to cross-metathesis, ADMET also involves the formation of metallacyclobutane intermediate by [2+2] cycloaddition of one of the double bonds of a terminal diene with...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Incidence and Correlation of Renal Pathologies Based on 14-Year Kidney Biopsy Material: A Retrospective Single-Centre Study in Poland.

Journal of clinical medicine·2026
Same author

Clinical Model Predicting Presence of Diabetic Nephropathy in Renal Biopsy Performed on Patients with Diabetes.

Journal of clinical medicine·2026
Same author

Constraint driven modulation of <i>N</i>,<i>N</i>-diarylamine(s) under space limitations imprinted in 7.6.6 defect.

Chemical communications (Cambridge, England)·2026
Same author

Spatiotemporal Variability of Dendroecological Indicators in Pedunculate Oak (Quercus robur L.) Tree-Rings Across Europe in Relation to Species Distribution Models.

Global change biology·2025
Same author

Rigidity vs Activity: Design of Gramicidin S Analogs against Multidrug-Resistant Bacteria Based on Molecular Engineering.

Journal of medicinal chemistry·2025
Same author

Prognostic Factors of Proteinuria Remission in Primary Membranous Nephropathy.

Journal of clinical medicine·2025

Related Experiment Video

Updated: May 14, 2025

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
07:53

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

Published on: August 16, 2019

9.2K

Affinity on Demand: A One-Pot Method for Synthesis and Sample Enrichment Using TentaGel-Functionalized Resins.

Michalina Zawadzka1, Wojciech Gil1, Andrzej Konieczny2

  • 1Faculty of Chemistry, University of Wrocław, Joliot-Curie 14, Wrocław 50-383, Poland.

ACS Omega
|May 12, 2025
PubMed
Summary

Researchers developed a novel resin for selectively enriching glycated peptides, crucial for understanding diabetes complications. This cost-effective method enhances proteomic analysis of these challenging analytes in complex biological samples.

More Related Videos

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.7K
Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
07:16

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

1.6K

Related Experiment Videos

Last Updated: May 14, 2025

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
07:53

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

Published on: August 16, 2019

9.2K
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.7K
Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
07:16

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

1.6K

Area of Science:

  • Biochemistry
  • Proteomics
  • Analytical Chemistry

Background:

  • Protein glycation, forming Amadori products, is implicated in diabetes complications.
  • Analyzing low-abundance glycated peptides in biological samples presents significant proteomic challenges.
  • Selective analyte enrichment is essential for overcoming detection limitations.

Purpose of the Study:

  • To develop a novel, efficient method for synthesizing functionalized resins for glycated peptide enrichment.
  • To evaluate the affinity and selectivity of the synthesized resins for glycated peptides.
  • To demonstrate the applicability of the resin in analyzing complex biological samples.

Main Methods:

  • A one-pot synthesis method for creating phenylboronic acid-functionalized resins with tunable properties.
  • Application of the highest-affinity resin (4PhB-3Lys-TGR) for selective enrichment of glycated peptides from albumin hydrolysate, patient serum, and milk.
  • Bioinformatic analysis to assess the coverage of protein sequences identified from enriched glycated peptides.

Main Results:

  • Successfully synthesized functionalized resins with high affinity for glycated peptides.
  • Demonstrated selective enrichment of glycated peptides from various complex biological matrices.
  • Bioinformatic analysis confirmed high protein sequence coverage, validating the enrichment method's effectiveness.

Conclusions:

  • A novel, fast, simple, and cost-effective one-pot method for synthesizing resins for selective glycated peptide enrichment has been developed.
  • The developed resin and enrichment method are effective for proteomic analysis of glycated peptides in complex samples.
  • The approach holds potential for affinity-based isolation of other compound classes, including Amadori products and carbonyl compounds.