Comprehensive Chemical Profiling of Moringa oleifera Leaves Extracts by LC-MS/MS Followed by In Silico ADMET...

Area of Science:

• Phytochemistry
• Pharmacognosy
• Analytical Chemistry

Background:

• Moringa oleifera is recognized for its nutritional and medicinal value.
• Understanding the influence of habitat on M. oleifera's phytochemical profile is crucial for its medicinal applications.
• Polyphenols in M. oleifera contribute significantly to its health benefits.

Purpose of the Study:

• To analyze the nutritional and medicinal properties of Moringa oleifera leaves.
• To develop a method for simultaneous polyphenol quantification in M. oleifera extracts.
• To investigate the impact of different habitats on the quality and polyphenolic profile of M. oleifera.

Main Methods:

• High-Performance Liquid Chromatography with Photodiode Array and Electrospray Ionization Mass Spectrometry (HPLC-PDA-ESI-MS) was employed for polyphenol analysis.
• A simultaneous quantification method for polyphenols was developed and validated.
• Phenolic compounds were extracted from M. oleifera leaves from the Tabelbala region, Bechar, Algeria.

Main Results:

• A total of 16 phenolic compounds were identified in the M. oleifera leaf extracts.
• Quercetin-3-O-glucoside (964.43 μg/g dry matter), kaempferol (839.71 μg/g dry matter), and rutin (835.51 μg/g dry matter) were the most abundant compounds.
• Gallic acid (496.14 μg/g dry matter) was exclusively found in the acetonic extract.

Conclusions:

• The study provides a comprehensive polyphenolic profile of M. oleifera leaves from Algeria, valuable for qualitative assessments and clinical applications.
• The developed quantitative analysis methodology can be integrated into quality assessment protocols for M. oleifera products.
• While identified compounds show potential for preventing drug interactions, poor bioavailability and efflux transport necessitate advanced formulation strategies for enhanced therapeutic efficacy.