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Updated: Jun 11, 2026

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Published on: June 1, 2017
Deciphering the lipid composition and metabolic patterns of cashew apple: A comprehensive lipidomic analysis
Huifang Ma1, Shaodong Zeng1, Haijie Huang2
1Agricultural Products Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524001, PR China.
Abstract:
To thoroughly analysis the lipid composition and regularities of distribution in different cashew apple varieties, the fatty acid composition and lipid composition of cashew apples were separated and identified by ultra-high performance liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry, and the overall differences and metabolic pathways of lipids in eight cashew apples were analyzed by OPLS-DA and metabolomics. The results showed that cashew apples were composed of 17 fatty acids (oleic acid and palmitic acid, etc.) and 320 lipids in eight cashew apples, among which 190 lipid compounds with VIP value greater than 1 were screened out, and the lipid compounds in different cashew apples were quite different. The content of total fatty acids and total sterols was highest in Bra4. The difference in lipid composition between Moz3 and Gua was the largest, and 67 lipid compounds with significant differences were found, which mainly existed in nine metabolic pathways (steroid biosynthesis and linoleic acid metabolism, etc.). This study provided a scientific basis for the variety identification and functional product development of cashew apples.
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