[Method of demonstrating and determining glyphosate in biological matter]

A method was worked out for the extraction of glyphosate from plant and biologic material originating from experimentally treated terrains, animals, and birds. The 'sensitivity' of a number of developers was comparatively studied--used either alone or in combination, choosing the most effective one. It was found that the most proper mobile phase consisted of ethanol : ammonia gas : distilled water in a 1:1:1 ratio and a 0.3 per cent alcohol solution of ninhydrin with a following heating in a kiln at 105 degrees C in the course of 3 to 5 min, with a use of aluminium plaques (Merck production). It is concluded that the method could be employed in all labs as it is highly specific (0.0004 mg glyphosate) and does not require special apparatus.