Assessment of Catalase Inhibition Under e-Beam Irradiation.

Area of Science:

• Biochemistry
• Radiation Biology
• Food Science

Background:

• Catalase is a key antioxidant enzyme, essential for decomposing hydrogen peroxide.
• Understanding radiation effects on enzymes is crucial for biological systems and food safety.

Purpose of the Study:

• To investigate the impact of 1 MeV accelerated electron irradiation on catalase activity.
• To explore a novel biodosimetry method for radiation dose assessment.

Main Methods:

• Enzyme activity was measured using spectrophotometry and the oxygen bubble method.
• Calibration curves were developed to correlate irradiation dose with catalase inhibition.
• The study used irradiation doses of 100 Gy and 1000 Gy.

Main Results:

• Catalase activity showed a dose-dependent decrease following irradiation.
• A 1.5-fold reduction in activity was observed at 100 Gy.
• A significant 40-fold decrease in activity occurred at 1000 Gy.

Conclusions:

• Irradiation induces structural modifications in catalase, leading to reduced activity.
• Oxygen bubble formation delay time can serve as an express biodosimetry tool.
• This method has potential applications in radiation safety and food quality control.