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Transcriptome Analysis of Pepper Leaves in Response to Tomato Brown Rugose Fruit Virus Infection
Boshen Zhang1, Donghai Wang2, Mangle Chen2
1College of Agriculture and Biotechnology, Zhejiang University, 866 Yu Hang Tang Road, Hangzhou 310058, China.
Plants (Basel, Switzerland)
|May 14, 2025
Summary
This study reveals how chili pepper plants respond to Tomato brown rugose fruit virus (ToBRFV) at the gene expression level. Researchers identified key genes involved in defense mechanisms, offering insights into chili pepper
Area of Science:
- Plant Pathology
- Molecular Biology
- Genomics
Background:
- Chili pepper (Capsicum annuum L.) is a vital crop facing threats from emerging viruses.
- Tomato brown rugose fruit virus (ToBRFV) significantly reduces chili pepper yield and quality.
- Understanding chili pepper's molecular response to ToBRFV is crucial for crop protection.
Purpose of the Study:
- To investigate the transcriptional changes in chili pepper leaves upon ToBRFV infection.
- To identify differentially expressed genes (DEGs) and their associated biological pathways.
- To provide a molecular basis for chili pepper's defense against ToBRFV.
Main Methods:
- RNA-sequencing (RNA-Seq) was employed to analyze gene expression profiles.
- Chili pepper leaves were infected with ToBRFV or mock-treated.
- Gene Ontology (GO) and KEGG pathway analyses were performed on identified DEGs.
- RT-qPCR was used to validate the expression patterns of selected DEGs.
Main Results:
- A total of 1468 differentially expressed genes (DEGs) were identified: 1366 upregulated and 102 downregulated.
- Enrichment analyses revealed DEGs are involved in defense response, reactive oxygen species, protein folding, and plant-pathogen interactions.
- RT-qPCR validation confirmed the reliability of the RNA-Seq data.
Conclusions:
- This study provides the first transcriptomic analysis of chili pepper response to ToBRFV.
- Identified DEGs offer insights into the molecular mechanisms underlying chili pepper's defense.
- Potential candidate genes for future research on ToBRFV-plant interactions were highlighted.
