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From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
Published on: January 13, 2017
Cytotoxic polyketides from the desert soil-derived fungus Preussia fleischhakii H231
Xiaomei Song1,2, Yu Li3, Weikang Li3
1Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, Kepala Batas, Penang, Malaysia. vlim@usm.my.
Abstract:
Three new heterodimeric polyketides, fleisine A-C (1-3), were obtained from the fermentation product of the desert soil-derived fungus Preussia fleischhakii H231. The structures of 1-3 were elucidated through an integrated analysis of spectroscopic data, while the absolute configurations were established by chemical hydrolysis followed by calculated electronic circular dichroism (ECD). Biological evaluation revealed that compounds 1 and 2 exhibited potent cytotoxicity against multiple human tumor cell lines. The IC50 values ranged between 2.3 and 25.2 μM. Further in vitro assays demonstrated that compounds 1 and 2 significantly suppressed the proliferation and migration of gastric adenocarcinoma cells (SGC-7901 and MGC-803) dose-dependently. These findings demonstrate the potential of 1 and 2 as favorable candidates for further anticancer drug development.
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