Some molecular features of human cervical mucus

1) The time preservation effect of the enzymatic activities present in human cervical mucus has been studied. 2) In order to protect the enzymatic activities of the mucus from bacterial impurities, the enzymes studied were assayed in presence of NaN3. 3) The effect of an acid pH likes present in the vagina was also studied: a low pH exerts a marked inhibition on mucus enzymes. 4) the use of nonionic detergents for mucus solubilization does not produce a marked increase of the enzymatic activities studied. 5) A NADP-dependent malate dehydrogenase in peri- and post-ovulatory period has been identified. 6) The presence in mucus of at least four lactate dehydrogenase isoenzymes has been demonstrated and their absolute values determined.