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Plasmid DNA Cleavage Assay with Eukaryotic Topoisomerase II

Allison J Thomas1, Brooke D Latham1, Addison K O'Brian1

  • 1Department of Biological, Physical, and Human Sciences, Freed-Hardeman University, Henderson, TN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 15, 2025
PubMed

Keywords:
CleavageDNAGel electrophoresisPlasmidTopoisomerase II

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DNA Topoisomerases

Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
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