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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: May 16, 2025

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Published on: August 15, 2013

Analyzing Topoisomerase II Cleavage Complexes Using Flow Cytometry.

Maria Camila Gosso¹, Néstor R Aznar¹, Marcela B González-Cid¹

¹Laboratorio de Mutagénesis, Instituto de Medicina Experimental (IMEX), CONICET-ANM, Ciudad Autónoma de Buenos Aires, Argentina.

Methods in Molecular Biology (Clifton, N.J.)

|May 15, 2025

Summary

This summary is machine-generated.

We developed a novel flow cytometry method to rapidly analyze drug-induced topoisomerase II cleavage complexes (Top2cc) in human cells. This technique aids in high-throughput drug screening and studying DNA-related pathways.

Keywords:

Cell cycle Flow cytometry High-throughput examination Quantification method Top2 cleavage complex Top2 poisons Topoisomerase II

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Area of Science:

Cell Biology
Molecular Biology
Pharmacology

Background:

Flow cytometry is a key technique for analyzing cell populations.
Topoisomerase II (Top2) is a crucial enzyme involved in DNA replication and repair.
Drug-induced Top2 cleavage complexes (Top2cc) are important targets for cancer therapy.

Purpose of the Study:

To develop a flow cytometry-based method for analyzing drug-induced Top2cc.
To enable rapid, high-throughput analysis of Top2-targeting drugs.
To study the impact of Top2cc on cellular processes.

Main Methods:

Utilized flow cytometry for cellular analysis.
Developed a specific assay for detecting Top2cc.
Applied the method to various human cell types, including clinical samples.

Main Results:

The method allows rapid quantification of Top2cc in a cell cycle-dependent manner.
It can differentiate between Top2 isoforms and track drug removal.
The technique is applicable to diverse human cell types.

Conclusions:

This flow cytometry method provides a valuable tool for high-throughput screening of Top2-targeting drugs.
It facilitates the study of drug effects on DNA-related nuclear processes.
The methodology supports research in cancer therapy and drug development.