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Ultrasensitive and selective vancomycin detection using aptamer-modified multi-doped laser-induced graphene
Xinjie Li1, Linping Hu1, Feng Xu2
1School of Chemistry and Molecular Engineering, Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, Dongchuan Road 500, Shanghai, 200241, China.
Talanta
|May 15, 2025
Summary
A novel extended-gate field-effect transistor (EG-FET) sensor utilizing multi-doped graphene detects vancomycin with high sensitivity. This portable biosensor accurately measures vancomycin levels in blood, aiding precision medicine.
Area of Science:
- Biosensor technology
- Nanomaterials in diagnostics
- Antibiotic monitoring
Background:
- Vancomycin is crucial but requires precise monitoring due to its narrow therapeutic window.
- Existing methods for vancomycin detection can be complex and time-consuming.
- Accurate vancomycin level monitoring is essential for patient safety and treatment efficacy.
Purpose of the Study:
- To engineer a novel extended-gate field-effect transistor (EG-FET) sensor for sensitive and selective vancomycin detection.
- To develop a portable and reliable platform for real-time vancomycin concentration monitoring.
- To validate the sensor's performance in detecting vancomycin in patient blood samples.
Main Methods:
- Fabrication of a multi-doped graphene extended-gate electrode using laser-induced graphene (LIG) integrated with MnO2 and Au nanoparticles.
- Immobilization of vancomycin aptamers onto the electrode surface for specific molecular recognition.
- Integration of the EG-FET sensor with a portable wireless system and a Janus membrane for plasma separation.
Main Results:
- The EG-FET sensor demonstrated high sensitivity and selectivity for vancomycin detection.
- A wide linear response range from 1 nM to 100 μM was achieved, with a low detection limit of 0.187 nM.
- The sensor successfully detected vancomycin concentrations in actual patient blood samples, showing reliable results.
Conclusions:
- The developed multi-doped graphene EG-FET sensor offers a promising tool for precise vancomycin monitoring.
- The portable sensing platform has significant potential for clinical applications and advancing precision medicine.
- This technology facilitates accurate antibiotic level assessment, improving therapeutic outcomes and patient care.
