Related Experiment Video
Updated: May 17, 2025
Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq
Published on: April 19, 2013
Single Nucleotide Variants of the SLC2A9 Gene Are Associated with Hyperuricemia in Mexican Patients with Type 2
Gloria Elizabeth Vázquez-Rivera1, Erika F Gómez-García2, Renato Parra-Michel3
1División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, Mexico.
Background:
Chronic kidney disease (CKD) due to diabetes is the third leading cause of death in Mexico. A relationship between high serum uric acid (SUA) levels and kidney disease have been demonstrated. On the other hand, variants of the SLC2A9 gene have been associated with elevated SUA concentrations. This study evaluated the associations of the rs11722228, rs3775948, rs7678287, and rs1014290 variants with hyperuricemia and biochemical parameters in patients with type 2 diabetes (T2D).
Methods:
The study included 1036 Mexican subjects with T2D. Patients were grouped based on the presence (n = 462) or absence (n = 574) of diabetic kidney disease (DKD). SUA concentrations and biochemical parameters were determined. Samples were genotyped through real-time polymerase chain reaction. Associations between genetic variants and hyperuricemia were analyzed by univariate and multivariate models adjusting for covariates.
Results:
SUA levels and hyperuricemia prevalence were higher in the DKD group. rs3775948 and rs1014290 showed negative associations with hyperuricemia in the whole sample and in patients without DKD. rs7678287 was positively correlated with SUA and hyperuricemia in patients without DKD, whereas rs11722228 was a risk factor only in the DKD group. The presence of DKD and elevated triglycerides and total cholesterol were significant factors associated with hyperuricemia.
Conclusions:
Variants rs3775948 and rs1014290 exhibit protective effects, while rs7678287 and rs11722228 may confer increased risk of hyperuricemia in Mexican patients with T2D. Analysis of SLC2A9 gene variants could help in detecting patients at increased risk of kidney or cardiovascular complications due to hyperuricemia.
Related Concept Videos
01:27Glucose Transporters
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
01:05Single Nucleotide Polymorphisms-SNPs
01:11Genome-wide Association Studies-GWAS
GWAS does not require the identification of the target gene involved in...
02:26Comparing Copy Number Variations and SNPs
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
01:33Pleiotropy
01:31Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of...