Single-cell sequencing and spatial transcriptomics reveal FAS+ T cell and autophagy-related signatures predicting...

Current subtyping methods of diffuse large B-cell lymphoma (DLBCL) could not satisfy the clinical demands for risk assessment and prognostic prediction. We aimed to investigate the prognostic effect of autophagy-related genes (ARGs) in DLBCL. Transcriptomic data of 1,409 DLBCL patients, 531 healthy controls (HCs), and single-cell sequencing data of 4 DLBCL were included. Validation involved spatial transcriptomics from 10 DLBCL patients and 110 DLBCL proteomic data from a local cohort. We identified 153 differentially expressed ARGs between DLBCL patients (n=48) and HCs (n=531), classifying 414 DLBCL patients into two subtypes based on autophagy heterogeneity. Subtype I, characterized by upregulated T regulatory (Treg) cells (P<0.0001) and T follicular helper (Tfh) cells (P=0.0012), showed a superior prognosis (P=0.035). Eight prognostic ARGs were selected to construct an autophagy-related model, dividing patients into low- and high-risk groups. Kaplan-Meier survival analysis revealed significantly better outcomes for the low-risk group in both the discovery (P<0.0001) and validation cohorts (P=0.0041). High-risk patients exhibited elevated IDO1 (P=0.042) and LAG3 (P<0.001) levels. Among the eight signature proteins, higher FAS was further verified to indicate a better prognosis in the local cohort (n=110) using antibody array (P=0.0083). FAS was primarily expressed in T cells such as Treg and Tfh cells and was elevated in non-progressive disease patients. FAS-positive T cells showed increased interferon-gamma (normalized enrichment score (NES)=2.196, FDR<0.0001) and alpha (NES=1.836, FDR<0.01) response activities. We constructed an autophagy-related model and identified FAS as a prognostic biomarker. FAS+ Treg and Tfh cell-enriched TME indicated a favorable prognosis.