Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Fluoroquinolones as the linchpin of tuberculosis therapy: contrasting efficacy and resistance profiles in <i>Mycobacterium tuberculosis</i> versus <i>Mycobacterium abscessus</i>.

Frontiers in cellular and infection microbiology·2026

Same author

Breast milk miRNAs and their potential role in the development of atopy in infants.

Frontiers in molecular biosciences·2026

Same author

Regulatory Potential of piRNAs Targeting <i>Klotho</i> and Other Genes.

Genes·2026

Same author

Plant-Derived miRNAs as Potential Cross-Kingdom Cancer Regulators.

Genes·2025

Same author

The Fine Structure of Genome Statistics-The Frequency and Size.

Life (Basel, Switzerland)·2025

Same author

In Silico Analysis of miRNA-mRNA Binding Sites in <i>Arabidopsis thaliana</i> as a Model for Drought-Tolerant Plants.

Plants (Basel, Switzerland)·2025

Search research articles

Related Experiment Video

Updated: Jun 29, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

An Overview of miRNA and miRNA Target Analysis Tools.

Aizhan Rakhmetullina¹, Anna Lukasik¹, Piotr Zielenkiewicz^2,3

¹Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.

Methods in Molecular Biology (Clifton, N.J.)

|May 16, 2025

Summary

This summary is machine-generated.

This chapter reviews computational tools for plant microRNA (miRNA) analysis, including new databases and updated features on the Tools4miRs platform. These resources aid in understanding miRNA roles in plant biology and disease.

Keywords:

miRNA, Analysis, Tools, Bioinformatics, Target gene, Prediction, Tools4miRs

More Related Videos

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

mirMachine: A One-Stop Shop for Plant miRNA Annotation

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

Related Experiment Videos

Last Updated: Jun 29, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

mirMachine: A One-Stop Shop for Plant miRNA Annotation

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

Area of Science:

* Plant molecular biology
* Bioinformatics
* Genomics

Background:

* MicroRNAs (miRNAs) are crucial regulators in diverse biological processes.
* Growing interest in miRNAs necessitates advanced computational analysis tools.
* Existing tools support various miRNA-related analyses like detection and target prediction.

Purpose of the Study:

* To provide an overview of computational tools for plant miRNA analysis.
* To introduce new databases and updated features within the Tools4miRs platform.
* To enhance accessibility to current research findings for plant miRNA studies.

Main Methods:

* Review and curation of existing computational tools for miRNA analysis.
* Development and integration of new small noncoding RNA sequence databases.
* Updating existing tools with comprehensive references and accessible links.

Main Results:

* An updated overview of plant miRNA analysis tools is presented.
* New databases with extensive small noncoding RNA sequence information have been added to Tools4miRs.
* Several Tools4miRs components now feature updated references and links.

Conclusions:

* The Tools4miRs platform offers enhanced resources for plant miRNA research.
* Updated tools and databases facilitate more comprehensive and current miRNA analyses.
* These advancements support researchers in exploring miRNA functions in plants.