Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distinct Resting-State Functional Connectivity Profiles in ADHD with and without Prenatal Alcohol Exposure.

medRxiv : the preprint server for health sciences·2026
Same author

Anterior middle cingulate cortex gamma-aminobutyric acid level is elevated in children with both familial and prenatal alcohol exposure-associated attention deficit hyperactivity disorder.

medRxiv : the preprint server for health sciences·2026
Same author

Implementation of risk-stratified tuberculosis screening in psoriasis patients receiving biologic therapy: A retrospective study from an academic medical center.

Journal of the American Academy of Dermatology·2026
Same author

Supraventricular tachycardia owing to 2-for-1 atrioventricular nodal conduction in a patient with repaired tetralogy of Fallot.

HeartRhythm case reports·2026
Same author

Spermine Oxidase Serves as a Key Functional Node in Microbial Dysbiosis-Induced Breast Carcinogenesis.

Cancer research·2026
Same author

Genetic and Phenotypic Features of the Five Known Polyaminopathies: A Critical Narrative Review.

American journal of medical genetics. Part A·2025

Related Experiment Video

Updated: May 20, 2025

Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS
09:20

Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS

Published on: August 10, 2017

8.5K

Cell-based determination of HDAC10-mediated polyamine deacetylase activity.

Ishika Gupta1, Ashley Nwafor1, Robert A Casero1

  • 1Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins School of Medicine, Baltimore, MD, United States.

Methods in Enzymology
|May 17, 2025
PubMed
Summary

Histone deacetylase 10 (HDAC10) uniquely processes N8-acetylspermidine, aiding polyamine homeostasis. New cell-based assays quantify this polyamine deacetylase (PDAC) activity for research and drug screening.

Keywords:
HDAC10 assayHistone deacetylase 10N8-acetylspermidinePolyamine deacetylaseSpermidine metabolism

More Related Videos

Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

6.5K
Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
09:07

Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay

Published on: May 1, 2019

6.7K

Related Experiment Videos

Last Updated: May 20, 2025

Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS
09:20

Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS

Published on: August 10, 2017

8.5K
Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

6.5K
Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
09:07

Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay

Published on: May 1, 2019

6.7K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Histone deacetylases (HDACs) are crucial enzymes involved in epigenetic regulation.
  • HDAC10 exhibits a unique substrate specificity for acetylated polyamines, particularly N8-acetylspermidine (N8-AcSpd).
  • N8-AcSpd is an extracellular metabolite produced by certain intestinal microbes, influencing cellular polyamine levels.

Purpose of the Study:

  • To detail methodologies for quantifying HDAC10-mediated polyamine deacetylase (PDAC) activity.
  • To establish cell-based assays for assessing tissue-specific PDAC activity.
  • To provide a foundation for high-throughput screening of HDAC10 modulators.

Main Methods:

  • Development of cell-based assays to measure relative levels of HDAC10-mediated PDAC activity.
  • Utilizing N8-AcSpd as a specific substrate for HDAC10.
  • Cost-efficient experimental design for broad applicability.

Main Results:

  • HDAC10's role as a specific polyamine deacetylase is confirmed.
  • The assays allow for the determination of tissue-specific differences in PDAC activity.
  • The methods are adaptable for screening potential HDAC inhibitors.

Conclusions:

  • HDAC10 plays a key role in polyamine homeostasis through its unique deacetylase activity.
  • Quantifying PDAC activity is essential for understanding tissue-specific polyamine metabolism.
  • The developed assays offer a valuable tool for pharmacological research targeting HDAC10.