Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Compartmental analysis: theoretical aspects and application.

H G Holzhütter

Biomedica Biochimica Acta

|January 1, 1985

Summary

This study presents methods for solving the inverse problem in metabolic systems analysis. It offers analytical solutions for isotopic tracing in the citric acid cycle and kinetic analysis of simultaneous reactions, like malaria parasite glycolysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Study on UV Filter Chemicals from Annex VII of European Union Directive 76/768/EEC, in the In Vitro 3T3 NRU Phototoxicity Test.

Alternatives to laboratory animals : ATLA·2015

Same author

The ECVAM Prevalidation Study on the Use of EpiDerm for Skin Corrosivity Testing.

Alternatives to laboratory animals : ATLA·2014

Same author

Modeling function-perfusion behavior in liver lobules including tissue, blood, glucose, lactate and glycogen by use of a coupled two-scale PDE-ODE approach.

Biomechanics and modeling in mechanobiology·2014

Same author

EEC/COLIPA project on in vitro phototoxicity testing: First results obtained with a Balb/c 3T3 cell phototoxicity assay.

Toxicology in vitro : an international journal published in association with BIBRA·2010

Same author

The International EU/COLIPA In Vitro Phototoxicity Validation Study: Results of Phase II (Blind Trial). Part 1: The 3T3 NRU Phototoxicity Test.

Toxicology in vitro : an international journal published in association with BIBRA·2010

Same author

The ECVAM International Validation Study on In Vitro Tests for Skin Corrosivity. 2. Results and Evaluation by the Management Team.

Toxicology in vitro : an international journal published in association with BIBRA·2010

Area of Science:

Biochemistry
Systems Biology
Metabolic Engineering

Background:

Metabolic compartmental analysis involves complex kinetic modeling.
The inverse problem, estimating model parameters from experimental data, is a significant challenge.
Understanding metabolic fluxes in different physiological spaces is crucial for disease research.

Purpose of the Study:

To present basic equations for metabolic compartmental systems kinetics.
To address the inverse problem in compartmental analysis using two distinct examples.
To develop theoretical approaches for estimating kinetic parameters in complex biological systems.

Main Methods:

Matrix formulation for analytical solutions in compartmental systems.
Application of theoretical approaches to the citric acid cycle in rabbit reticulocytes.
Development of methods to estimate separate flux rates in simultaneous biochemical reactions using total rates and metabolite concentrations.

Main Results:

An analytical solution approach for isotopic carbon distribution in the citric acid cycle was outlined.
The study demonstrated an application to the citric acid cycle of rabbit reticulocytes.
A theoretical approach was proposed for differentiating kinetic rates in host-parasite metabolic pathways.

Conclusions:

The presented methods offer a framework for solving the inverse problem in metabolic analysis.
The approaches are applicable to complex systems like the citric acid cycle and host-pathogen interactions.
This work contributes to the kinetic characterization of metabolic pathways in distinct physiological compartments.

Related Experiment Videos