Screening of β-Thalassemia Carriers based on Matrix Assisted Laser Desorption Ionization Time-Of-Flight Mass
Background:
The traditional analytical methods for β-thalassemia carriers are time-consuming and costly. This study takes advantage of matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to directly analyze human whole blood samples and established a preliminary method for screening β-thalassemia carriers.
Methods:
A sample of 3 µL whole blood was diluted 200-fold with distilled water, mixed with sinapic acid, and subjected to MALDI-TOF MS analysis. The efficacy of the method for the screening of β-thalassemia carriers was assessed using the β-thalassemia gene results as the standard.
Results:
β-thalassemia gene results showed 109 β-thalassemia carriers and 110 normal individuals out of 219 samples. We developed a δ-globin peak slope index that can simply and effectively distinguish β-thalassemia carriers from healthy individuals, the best cutoff value for the slope index was -0.819.
Conclusions:
MALDI-TOF MS has the potential to be a valuable tool for β-thalassemia carriers screening.
More Related Videos
07:49Matrix-assisted Laser Desorption/Ionization Time of Flight MALDI-TOF Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
Published on: September 9, 2013
09:31Visualization of Amyloid β Deposits in the Human Brain with Matrix-assisted Laser Desorption/Ionization Imaging Mass Spectrometry
Published on: March 7, 2019
Related Concept Videos
01:08Matrix-Assisted Laser Desorption Ionization (MALDI)
The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix material. The...
01:33Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...