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Related Experiment Video

Updated: May 21, 2025

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Published on: December 22, 2017

Generating Primary Cultures for Keratinocyte Live Cell Imaging.

Brook Abegaze¹, Nwamaka Ijeh², Brooke Vittimberga²

¹The University of California, San Francisco; San Francisco Veterans Affairs Health Care System.

Journal of Visualized Experiments : Jove

|May 19, 2025

Summary

This summary is machine-generated.

This study presents a new live-cell imaging protocol for tracking human skin stem cells and progenitors. The method allows detailed observation of individual cell behavior and proliferation in vitro.

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Area of Science:

Dermatology
Cell Biology
Stem Cell Research

Background:

Studying human skin stem cells and progenitor behavior at the single-cell level is challenging.
Live-cell imaging offers advancements but presents technical difficulties in vitro.

Purpose of the Study:

To develop a protocol for culturing and monitoring individual human keratinocyte stem cells and progenitors.
To enable long-term time-lapse photography for observing cell division and behavior.

Main Methods:

Culturing primary human keratinocytes at low seeding density (clonal density).
Utilizing time-lapse photography for monitoring individual cell divisions and behavior.
Focusing on proliferation but adaptable for migration, wound healing, and motility studies.

Main Results:

The protocol enables the culture of keratinocytes at clonal density for extended observation.
Individual cell divisions and their timing can be accurately documented.
The method provides a foundation for studying various aspects of keratinocyte behavior in vitro.

Conclusions:

This protocol facilitates detailed, single-cell level analysis of human keratinocyte behavior in vitro.
It offers a valuable tool for understanding stem cell and progenitor dynamics.
The adaptable method supports diverse live-cell imaging applications in skin research.