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Updated: Jun 13, 2025
Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog
Published on: November 5, 2014
Discovery and evaluation of a novel selective fluorescent substrate for CYP3A4 based on sulfoxidation characteristics
Chao-Hua Yan1, Gui-Lin Wei1,2, Zhuo-An Jin1
1Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China. chemzlw@163.com.
Abstract:
This study for the first time reports the design and application of a novel type of selective fluorescent substrate NESF for cytochrome P450 3A4 (CYP3A4) based on sulfoxidation reaction. NESF has been successfully applied for imaging endogenous CYP3A4 in living cells and for high-throughput screening of CYP3A4 inhibitors.
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