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Updated: May 7, 2026
08:59
Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
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A New Phospholipase D-Producing Bacillus cereus: Taxonomy, Mutagenesis, Fermentation Optimization and Enzyme
1Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.
Applied Biochemistry and Biotechnology
|May 20, 2025
Summary
Bacillus cereus was engineered to produce enhanced Phospholipase D (PLD) enzyme. This improved enzyme efficiently converts phosphatidylcholine (PC) to phosphatidylserine (PS) for industrial applications.
Area of Science:
- Biotechnology
- Enzyme Engineering
- Microbial Fermentation
Background:
- Phospholipase D (PLD) is crucial for industrial phosphatidylcholine (PC) to phosphatidylserine (PS) conversion.
- Identifying and enhancing microbial PLD sources is vital for biotechnological applications.
Purpose of the Study:
- To isolate and enhance PLD-producing Bacillus cereus strains.
- To optimize fermentation conditions for maximum PLD activity.
- To evaluate the enzyme's stability and kinetic properties for PS production.
Main Methods:
- Soil screening for PLD-producing Bacillus cereus.
- Mutagenesis strategies (chemical, irradiation) to enhance PLD activity.
- Response Surface Methodology (RSM) for fermentation optimization.
- Enzyme characterization (stability, kinetics) and bioconversion assays.
Main Results:
- A high-activity mutant (CI 7-12) showed a 312.2% increase in PLD activity.
- Optimized fermentation yielded 35 U/mL PLD activity.
- The enzyme exhibited broad stability (30-60°C, pH 6-10) and a long half-life (128 days).
- Bioconversion achieved 53.0% PC to PS conversion with 92.3% selectivity.
Conclusions:
- Engineered Bacillus cereus provides a robust source of enhanced PLD.
- Optimized conditions and enzyme properties support efficient PS production.
- This study highlights the potential of microbial PLD in industrial bioconversion processes.
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