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Melatonin regulates proline metabolism by modulating CmDREB1A/E to enhance cold tolerance in cantaloupe fruit
Shuai Huang1, Ying Bi1, Shifeng Cao1
1College of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi, 830052, China.
Abstract:
The potential regulatory mechanisms of 100 microM melatonin (MT) treatment on proline accumulation in cantaloupe fruit under cold stress were investigated. The results showed that MT treatment increased the content of free proline in cold-stored cantaloupe fruit by upregulating the expressions of the Δ1-pyrroline-5-carboxylate synthase (P5CS) and ornithine δ-aminotransferase (OAT) genes and decreasing the transcripts and enzyme activity of proline dehydrogenase (ProDH). Furthermore, the yeast one-hybrid assay screened for CmDREB1A and CmDREB1E that bind CmP5CS, and electrophoretic mobility shift analysis and dual luciferase reporter gene verified that these two transcription factors could bind the CmP5CS promoter. Further experiments showed that CmDREB1A and CmDREB1E were involved in improving cold tolerance in cantaloupe by MT and regulating the expression of CmP5CS. Our study suggests that MT treatment promotes CmP5CS expression and positively regulates proline accumulation by activating CmDREB1A and CmDREB1E in cantaloupe, thereby improving fruit cold stress tolerance.
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