From structure to function: Decoding peptidoglycan O-acetylation in pathogenic bacteria

Numerous pathogenic and non-pathogenic bacteria modulate the structure of their cell wall to escape the action of lytic enzymes that target it, threatening cell integrity. Of these modifications, the most taxonomically widespread is the addition of an acetyl to the C6 hydroxyl group of muramyl residues within the essential cell-wall heteropolymer peptidoglycan. This modification is found in many clinically important pathogens, including the WHO priority pathogens Neisseria gonorrhoeae, Staphylococcus aureus, Enterococcus faecium, and Streptococcus pneumoniae. In this review, we summarize the last 60 years of discoveries about the genetics, biochemistry, structural biology, and cellular metabolism that underlie this enigmatic bacterial self-defence mechanism.