Related Experiment Video
Updated: May 23, 2025

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization
Published on: June 28, 2012
Chromosome-level genome assembly of the predatory flower bug Orius strigicollis
Yaru Wang1,2, Limin Chen2,3, Farman Ullah2
1Xianghu Laboratory, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Abstract:
Predatory insects serve as important biological control agents in ecosystems by efficiently reducing pest populations. Orius strigicollis is a highly effective beneficial predator in cultivated fields in China. It is widely used as a biological control agent against many pests, including thrips, aphids, and spider mites. The lack of public availability of O. strigicollis genomes has limited our ability to thoroughly explore and research its evolution and gene function, particularly genes related to pesticide resistance. Here, we report a chromosome-scale assembly of the O. strigicollis genome by PacBio High fidelity (HiFi) long-reads, high-throughput chromosome conformation capture (Hi-C) and Illumina sequencing technologies. The final genome assembly was 167 Mb with a scaffold N50 size of 12.49 Mb, and 97.87% of the assembly was anchored to 12 chromosomes. Annotation identified 11,551 protein-coding genes. In this article, we reported a high-quality chromosome-scale assembly genome for the species of the genus Orius for the first time, which provide valuable resources for functional gene research and genetic transformation of natural enemy insects.
More Related Videos
12:10An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
Published on: November 30, 2014
07:20Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions
Published on: August 31, 2018
Related Concept Videos
Lampbrush Chromosomes
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...
Genome Annotation and Assembly
Heterochromatin
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
Evolutionary Relationships through Genome Comparisons