[Effect of certain factors on sheep oocyte maturation when cultured within intact follicles]

The effect of the medium composition and cultivation time on the maturation of sheep oocytes cultivated in vitro within the intact follicles was studied. The highest incidence of maturation was observed in medium 199 with 20% of sheep serum taken from an animal on the 16th day of the sexual cycle in a gaseous mixture of the following composition: 5% O2, 5% CO2, 90% N2 during 24 hrs.