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Updated: Jul 29, 2026
An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
Published on: November 30, 2014
Genome-wide cataloging and orthology analysis of long noncoding RNA expression in three species of Anopheles mosquito
Waseem Akram Malla1, Kuldeep Singh2,3, Niha Ayman4
1ICMR-National Institute of Malaria Research, Field Unit Guwahati, Guwahati, Assam, 781036, India. malla.wa@icmr.gov.in.
Background:
Long noncoding RNAs (lncRNAs) are versatile regulatory molecules that affect cellular phenotypes through context-specific expression. While their role in controlling cellular pathways is well-established in insects, investigating lncRNA expression in Anopheles in a tissue- and species-specific manner could add to our understanding of malaria transmission by this important vector.
Methodology:
We performed de novo transcriptome assembly of Anopheles minimus, Anopheles albimanus, and Anopheles arabiensis utilising publicly available RNA-Seq datasets of male reproductive tissues, male carcasses, female reproductive tissues, and female carcasses. Various bioinformatics tools were subsequently used for lncRNA identification, conservation analysis, and differential expression analysis across sexes and tissues.
Results:
We identified 9331, 5372, and 5256 lncRNA transcripts in An. albimanus, An. arabiensis, and An. minimus, respectively. Compared with An. albimanus lncRNAs, conservation analysis revealed that a total of 1964 and 1400 lncRNAs were conserved in An. arabiensis and An. minimus; however, only 283 and 253 lncRNAs presented sequence-level conservation. Differential expression (DE) analysis revealed that the carcasses presented the lowest difference in lncRNA expression, whereas in each comparison, the reproductive tissues (whether male or female) presented relatively high levels of differential expression. Additionally, 69 lncRNAs were found to be conserved at the sequence level in all 3 species. These lncRNAs were almost exclusively upregulated in males (reproductive tissue as well as carcasses) and exclusively downregulated in female carcasses.
Conclusions:
The genes in the vicinity of differentially expressed, conserved lncRNAs were found to be involved in critical pathways such as nuclear structure, chromatin remodelling, protein and RNA metabolism, and cell cycle in each of these species. Future studies on these lncRNAs can provide useful insights into how these functions control sexually-dimorphic physiological phenomena such as host-seeking and biting behaviour of female mosquitoes, blood meal metabolism, reproductive behaviour, and disease-carrying capacity.
