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Updated: Aug 6, 2026

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Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
Published on: August 20, 2012
Interaction of ethidium bromide with ribosomes. Absorption, fluorescence, circular dichroism and sedimentation
Biochimica Et Biophysica Acta
|May 3, 1977
Abstract:
It is shown in this work that the binding of ethidium bromide to yeast ribosomes occurs through intercalation in the double-stranded rRNA regions and produces changes in the ribosomes structure, yielding unfolded subparticles, and even partial separation of proteins from rRNA at very high binding ratios. The addition of Mg2+ prevents these structural changes, probably by partial inhibition of the dye binding.

