Graphene-Integrated Ultrathin Neural Probe for Multiregional Cortical Recordings

Electrophysiological measurement techniques are essential for understanding the functions of the central and peripheral nervous systems. Specifically, noninvasive neural probes, such as surface electrode arrays, provide stable electrophysiological recordings without eliciting an immunological response. However, the ability to capture complex interactions across multiple brain regions is limited by their localized recording site. Here, we present the "large-area NeuroWeb (LNW)", an ultrathin, minimally invasive neural probe designed for extensive cortical recording and stimulation. LNW consists of four recording areas, each containing 16-channel platinum electrodes interconnected by graphene networks. In vivo experiments of the mouse brain exhibit stable, high-quality single-unit spike recordings for up to 7 days post-surgery. Simultaneous high-resolution neural activity recordings are performed across left/right somatosensory cortex and cerebellum, simplifying the experimental procedure by eliminating the necessity for multiple synchronized probes, thus reducing tissue displacement and inflammation. Furthermore, whisker and electrical stimulations demonstrate that the LNW has precise and bidirectional connections with neurons for reliable, region-specific signal acquisition and activation. These findings highlight the capability of LNW to facilitate comprehensive and accurate mapping of neuronal dynamics, thereby advancing brain-machine interfaces and neural prostheses.