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Updated: May 4, 2026

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
Published on: August 20, 2017
From Single Stem Cells to an In Vitro Model of the Post-implantation Human Embryo: A Step-by-Step Guide
Seher Ipek Gassaloglu1, Monique Pedroza1, Berna Sozen2,3,4
1Department of Genetics, Yale School of Medicine, Yale University, New Haven, CT, USA.
Abstract:
Recent advances in three-dimensional (3D) modeling of post-implantation human embryos using human pluripotent stem cells (hPSCs) have revolutionized our ability to investigate this crucial yet enigmatic stage of development. Here we detail the generation of the human extra-embryoid (hEE), a 3D stem cell-based embryo model that uniquely captures key spatiotemporal events of peri-gastrulation development through the formation and co-development of post-implantation embryonic and extra-embryonic lineages, with high efficiency and robustness across genetic backgrounds. This chapter provides a detailed protocol for generating hEEs in vitro, including guidance on hPSC maintenance, expected cell morphology, troubleshooting strategies, and key culture techniques.
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