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Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
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Protocol for CRISPR-mediated deletion of cis-regulatory element in murine Th17 cells for in vivo assessment of

Xiancai Zhong1, Hongmin Wu1, Guanpeng Wang1

  • 1Department of Immunology & Theranostics, Arthur Riggs Diabetes and Metabolism Research Institute, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA.

STAR Protocols
|May 21, 2025
PubMed

Keywords:
CRISPRImmunologyMolecular Biology

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